Title & Categories
|Case Study Title:
||Use DNA Barcoding Techniques to Identify Flowering Plants in San Rafael National Park, Paraguay
||Adding barcodes to a large survey of a taxonomic group, Biotic inventory of multiple taxa in a region or habitat
||Terrestrial temperate forest, Terrestrial tropical forest
||c: Magnoliophyta, c: PLANTS
Investigate, identify and document flowering plants in a floristically rich, under researched and threatened part of the Atlantic Forest.
The study of flowering plants will be conducted at Ecosara Reserve in San Rafael National Park, Paraguay – an area of approximately 73,000 hectares.
Data will be collected for all angiosperms that are observed within the park. It is anticipated that data will be restricted to taking digital images, small samples of plant material suitable for DNA extraction and barcoding and that no plant specimens will be taken at this stage.
The number of species is difficult to quantify, but is crudely estimated at 180- 850; the calculation is derived from the Paraguay specimen total currently available from the Royal Botanic Gardens Herbarium on-line catalogue.
The project will promote the use of DNA barcodes as an identification key for plant species. Using barcodes will help minimise the impact on fragile ecosystems by reducing and / or eliminating the number of specimens ‘collected’ from their natural environment.
The project aims are to:
- Conduct a scientific investigation of flora in San Rafael National park and establish a biodiversity inventory.
- Establish a plant photographic database
- Design and develop identification keys for the plant groups
- Publish findings across a broad scientific and non-scientific community
- Ally Ecosara with conservation organisations in other countries.
This provides the opportunity to initiate a DNA barcoding project in Paraguay using DNA to identify the flowering plants in San Rafael National Park.
The aims of Ecosara support CBOL’s mission to promote the exploration and development of DNA barcoding as a global standard for species identification, specifically the following goals:
- involvement of researchers and users of barcode data from all regions of the world, especially those with high biodiversity
- rapid compilation of high-quality DNA barcode records in a public library
- participation of taxonomists and research organizations in all regions and countries
- use of barcode data for the benefit of science and society
- development of intellectual activity involving DNA barcode data in the wider academic community among diverse users throughout society
- greater awareness of DNA barcoding beyond taxonomic researchers.
Paraguay is home to a wealth of floristic diversity, yet there is a paucity of available data; most of the botanical collections and literature is over a hundred years old. Research is needed to investigate, identify, document and preserve the country’s under-studied flora.
ECOSARA, (Estación Ecológica de San Rafael) is a research station aimed at saving one of the world’s most important, but little known Atlantic Forest Reserves – San Rafael National Park in Paraguay.The Forest once covered about one million square kilometres of Paraguay, Brazil and Argentina. Today, only 7 to 10% remains. Conservation International describes the Atlantic Forest as one of the most important ecosystems on the planet being of “critical importance for global biodiversity” and, because of the level of threat and endemism, has selected it as a Biodiversity Hotspot.
Deforestation continues at a high rate, yet Eastern Paraguay retains a large area of the Upper Paraná region, much of it degraded and in dispersed fragments, placing it amongst the most endangered ecosystems in the world. Estimates indicate that 40% of the thousands of plant species in the Upper Paraná are endemic to the region with many known for their medicinal properties.
In 1992 the government, recognising the area as a top conservation priority in Paraguay, designated 73,000 hectares as San Rafael National Park. Located in the south eastern departments of Caazapá and Itapúa, it is the most complete example of the diverse species and is the largest continuous block of the Upper Paraná Atlantic Forest in Paraguay.
The project is at the preliminary stage, with no resources yet having been identified. Ecosara is a non-profit ecological station, staffed by professionals yet also heavily reliant on a constant stream of volunteers, so timescales are uncertain. The project can start when resources become available.
However, before data collection can proceed, it is necessary to ascertain whether or not actual plant specimens are required as voucher evidence; The Royal Botanic Gardens Kew will be contacted to discuss the feasibility of accepting photographic evidence in combination with the DNA barcode, their advice will be sought on what data needs to be captured and how it will be preserved and stored.
A decision has been reached on which loci should be designated as the universal DNA barcode for plants. Formal acceptance by CBoL is awaited. Data collection is not dependent on this acceptance.
Each plant will be photographed in situ using a digital camera; multiple images will be obtained to show sufficient plant characteristics enabling species identification; comprehensive data is needed to provide a photographic voucher for each specimen.
In order to create a barcoding identification key, DNA material needs to be extracted from plant specimens currently held in herbaria. The cooperation of Missouri Botanical Garden and Royal Botanic Gardens Kew will be sought for their collaboration in accomplishing this.